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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
23/09/2019 |
Actualizado : |
23/09/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
COUTO, D.; STRANSFELD, L.; ARRUABARRENA, A.; ZIPFEL, C.; LOZANO-DURÁN, R. |
Afiliación : |
DANIEL COUTO, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; LENA STRANSFELD, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CYRIL ZIPFEL, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ROSA LOZANO-DURÁN, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; Shanghai Institutes of Biological Sciences, Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, China. |
Título : |
Broad application of a simple and affordable protocol for isolating plant RNA. (Short Report). |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
BMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS. |
ISSN : |
1756-0500 |
DOI : |
10.1186/s13104-015-1119-7 |
Idioma : |
Inglés |
Notas : |
Article history: Published on: 16 April 2015. |
Contenido : |
Abstract View references (4)
Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.
© 2015 Couto et al.; licensee BioMed Central. |
Palabras claves : |
Affordable; Arabidopsis; Protocol; RNA; Tomato; Wheat. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13321/1/Couto-et-al.-2015.-BMC-Research-Notes.pdf
https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-015-1119-7
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Marc : |
LEADER 02324naa a2200277 a 4500 001 1060208 005 2019-09-23 008 2015 bl uuuu u00u1 u #d 022 $a1756-0500 024 7 $a10.1186/s13104-015-1119-7$2DOI 100 1 $aCOUTO, D. 245 $aBroad application of a simple and affordable protocol for isolating plant RNA. (Short Report).$h[electronic resource] 260 $c2015 500 $aArticle history: Published on: 16 April 2015. 520 $aAbstract View references (4) Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide. © 2015 Couto et al.; licensee BioMed Central. 653 $aAffordable 653 $aArabidopsis 653 $aProtocol 653 $aRNA 653 $aTomato 653 $aWheat 700 1 $aSTRANSFELD, L. 700 1 $aARRUABARRENA, A. 700 1 $aZIPFEL, C. 700 1 $aLOZANO-DURÁN, R. 773 $tBMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Treinta y Tres. |
Fecha actual : |
04/07/2018 |
Actualizado : |
14/12/2022 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
QUINTANS, G.; CLARIGET, J.M.; LÓPEZ MAZZ, C.; PIAGGIO, L.; BANCHERO, G. |
Afiliación : |
GRACIELA QUINTANS ILARIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN MANUEL CLARIGET BRIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS LÓPEZ MAZZ, Universidad de la República (UdelaR); Facultad de Agronomía, Uruguay.; LUCIA PIAGGIO, Secretariado Uruguayo de la Lana (SUL), Uruguay.; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Programación fetal. ¿La nutrición que reciben las vacas y ovejas durante su gestación afectan el desempeño de sus crías?. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. |
Páginas : |
p. 79-83. |
Idioma : |
Español |
Palabras claves : |
PROGRAMACIÓN FETAL DEL TEJIDO MUSCULAR; PROGRAMACIÓN FETAL EN BOVINOS; PROGRAMACIÓN FETAL EN OVINOS. |
Thesagro : |
BOVINOS PARA CARNE; FERTILIDAD; OVINOS; TASA OVULATORIA. |
Asunto categoría : |
L53 Fisiología Animal - Reproducción |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10680/1/BUIATRIA-2018-Quintans-1.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/12692/1/BUIATRIA-2018-Quintans-1.pdf
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Marc : |
LEADER 00950naa a2200253 a 4500 001 1058685 005 2022-12-14 008 2018 bl uuuu u00u1 u #d 100 1 $aQUINTANS, G. 245 $aProgramación fetal. ¿La nutrición que reciben las vacas y ovejas durante su gestación afectan el desempeño de sus crías?. 260 $c2018 300 $ap. 79-83. 650 $aBOVINOS PARA CARNE 650 $aFERTILIDAD 650 $aOVINOS 650 $aTASA OVULATORIA 653 $aPROGRAMACIÓN FETAL DEL TEJIDO MUSCULAR 653 $aPROGRAMACIÓN FETAL EN BOVINOS 653 $aPROGRAMACIÓN FETAL EN OVINOS 700 1 $aCLARIGET, J.M. 700 1 $aLÓPEZ MAZZ, C. 700 1 $aPIAGGIO, L. 700 1 $aBANCHERO, G. 773 $tIn: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018.
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INIA La Estanzuela (LE) |
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